Abietic acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling

Jung, Tae Woo; Jeong, Ju-Cheol; Park, Seung Yeon; Cho, Wonjun; Oh, Heeseung; Lee, Hyun Jung; Hacımüftüoğlu, Ahmet; Hassıbelnaby, Abdelaty Mostafa Abdelaty; Bang, Joon Seok; Jeong, Ji Hoon

doi:10.1016/j.bbrc.2022.04.010

Makale Tam Makale Uluslararası 2022

Abietic acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling

Tae Woo Jung, Ju-Cheol Jeong, Seung Yeon Park, Wonjun Cho, Heeseung Oh, Hyun Jung Lee, Ahmet Hacımüftüoğlu, Abdelaty Mostafa Abdelaty Hassıbelnaby, Joon Seok Bang, Ji Hoon Jeong

Biochemical and Biophysical Research Communications Cilt 608 · ss. 142–148 · 11.06.2022

DOI ile aç

Özet

© 2022 Elsevier Inc.Abietic acid (AA), the main component of pine resin that has been traditionally used as Asian medicine, has been reported to demonstrate anti-inflammatory activities. Despite this, little is known about the effects of AA on hepatic endoplasmic reticulum (ER) stress and lipid metabolism. This study investigated the impacts of AA on ER stress and steatosis in in vitro obesity models. We found that Treatment with AA reduced lipid deposition and lipogenesis-related proteins expression in human primary hepatocytes. Augmented expression of ER stress markers (phospho-eukaryotic initiation factor-2α (eIF2α) and C/EBP homologous protein (CHOP)) in palmitate-treated hepatocytes were reversed by AA treatment. Further, AA treatment increased the expression of phospho-AMPK and oxygen-regulated protein 150 (ORP150) in hepatocytes. siRNA-associated knockdown of AMPK or ORP150 expression reduced the effects of AA on not only hepatic ER stress but also lipogenesis and apoptosis. These results denote that AA attenuates lipid accumulation in hepatocytes in the presence of palmitate through the suppression of ER stress by AMPK/ORP150 signaling. AA could be a potential candidate for treating non-alcoholic fatty liver disease.

Anahtar kelimeler

ER stress NAFLD AMPK Abietic acid ORP150

Sürdürülebilir Kalkınma Amaçları

SKA 3: Sağlıklı Bireyler

Atıf için künye

Jung, T. W., Jeong, J., Park, S. Y., Cho, W., Oh, H., Lee, H. J., Hacımüftüoğlu, A., Hassıbelnaby, A. M. A., Bang, J. S., & Jeong, J. H. (2022). Abietic acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling. Biochemical and Biophysical Research Communications, 608, 142–148. https://doi.org/10.1016/j.bbrc.2022.04.010

BibTeX

@article{jung2022-209723,
  author = {Jung, Tae Woo and Jeong, Ju-Cheol and Park, Seung Yeon and Cho, Wonjun and Oh, Heeseung and Lee, Hyun Jung and Hacımüftüoğlu, Ahmet and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Bang, Joon Seok and Jeong, Ji Hoon},
  title = {Abietic acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling},
  journal = {Biochemical and Biophysical Research Communications},
  year = {2022},
  volume = {608},
  pages = {142--148},
  issn = {0006-291X},
  doi = {10.1016/j.bbrc.2022.04.010},
  url = {https://akademik.atauni.edu.tr/yayinlar/209723-abietic-acid-alleviates-endoplasmic-reticulum-stress-and-lipid-accumulation-in}
}