Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice

Wen, Jiaying; Chen, Shengwei; Bao, Minglong; Hu, Canying; Wu, Lianyun; Yong, Yanhong; Liu, Xiaoxi; Li, Youquan; Yu, Zhichao; Ma, Xingbin; Eun, Jong-Bang; Shim, Jae-Han; Warda, Mohamad Alı Ahmad; Hassıbelnaby, Abdelaty Mostafa Abdelaty; Ju, Xianghong

doi:10.1016/j.carbpol.2022.120179

Makale Tam Makale Uluslararası 2023

Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice

Jiaying Wen, Shengwei Chen, Minglong Bao, Canying Hu, Lianyun Wu, Yanhong Yong, Xiaoxi Liu, Youquan Li, Zhichao Yu, Xingbin Ma, Jong-Bang Eun, Jae-Han Shim, Mohamad Alı Ahmad Warda, Abdelaty Mostafa Abdelaty Hassıbelnaby, Xianghong Ju

CARBOHYDRATE POLYMERS Cilt 299 · 01.01.2023

DOI ile aç

Özet

The mechanism underlying the intestinal transport of COS is not well understood. Here, transcriptome and proteome analyses were performed to identify potential critical molecules involved in COS transport. Enrichment analyses revealed that the differentially expressed genes in the duodenum of the COS-treated mice were mainly enriched in transmembrane and immune function. In particular, B2 m, Itgb2, and Slc9a1 were upregulated. The Slc9a1 inhibitor decreased the transport efficiency of COS both in MODE-K cells (in vitro) and in mice (in vivo). The transport of FITC-COS in Slc9a1-overexpressing MODE-K cells was significantly higher than that in empty vector-transfected cells (P < 0.01). Molecular docking analysis revealed the possibility of stable binding between COS and Slc9a1 through hydrogen bonding. This finding indicates that Slc9a1 plays a crucial role in COS transport in mice. This provides valuable insights for improving the absorption efficiency of COS as a drug adjuvant.

Anahtar kelimeler

EXPRESSION CANCER GENE mice nanoparticles CHITIN EXCHANGER INTESTINE Chitosan oligosaccharide Transcriptome Transport molecule

Atıf için künye

Wen, J., Chen, S., Bao, M., Hu, C., Wu, L., Yong, Y., Liu, X., Li, Y., Yu, Z., Ma, X., Eun, J., Shim, J., Warda, M. A. A., Hassıbelnaby, A. M. A., & Ju, X. (2023). Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice. CARBOHYDRATE POLYMERS, 299. https://doi.org/10.1016/j.carbpol.2022.120179

BibTeX

@article{wen2023-209719,
  author = {Wen, Jiaying and Chen, Shengwei and Bao, Minglong and Hu, Canying and Wu, Lianyun and Yong, Yanhong and Liu, Xiaoxi and Li, Youquan and Yu, Zhichao and Ma, Xingbin and Eun, Jong-Bang and Shim, Jae-Han and Warda, Mohamad Alı Ahmad and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Ju, Xianghong},
  title = {Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice},
  journal = {CARBOHYDRATE POLYMERS},
  year = {2023},
  volume = {299},
  issn = {0144-8617},
  doi = {10.1016/j.carbpol.2022.120179},
  url = {https://akademik.atauni.edu.tr/yayinlar/209719-slc9a1-plays-a-vital-role-in-chitosan-oligosaccharide-transport-across-the}
}