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A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Ge Chen, Rongqi Zhai, Guangyang Liu, Xiaodong Huang, Kaige Zhang, Xiaomin Xu, Lingyun Li, Yanguo Zhang, Jing Wang, Maojun Jin, Donghui Xu, Abdelaty Mostafa Abdelaty Hassıbelnaby

Frontiers in nutrition Cilt 9 · 07.02.2022
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Özet

Copyright © 2022 Chen, Zhai, Liu, Huang, Zhang, Xu, Li, Zhang, Wang, Jin, Xu and Abd El-Aty.Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.

Anahtar kelimeler

BIOSENSOR pesticide residue aptamer carbendazim Au@Pt-DNA

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

Chen, G., Zhai, R., Liu, G., Huang, X., Zhang, K., Xu, X., Li, L., Zhang, Y., Wang, J., Jin, M., Xu, D., & Hassıbelnaby, A. M. A. (2022). A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products. Frontiers in nutrition, 9. https://doi.org/10.3389/fnut.2022.820150

BibTeX 
@article{chen2022-209665,
  author = {Chen, Ge and Zhai, Rongqi and Liu, Guangyang and Huang, Xiaodong and Zhang, Kaige and Xu, Xiaomin and Li, Lingyun and Zhang, Yanguo and Wang, Jing and Jin, Maojun and Xu, Donghui and Hassıbelnaby, Abdelaty Mostafa Abdelaty},
  title = {A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products},
  journal = {Frontiers in nutrition},
  year = {2022},
  volume = {9},
  issn = {2296-861X},
  doi = {10.3389/fnut.2022.820150},
  url = {https://akademik.atauni.edu.tr/yayinlar/209665-a-competitive-assay-based-on-dual-mode-au-at-pt-dna-biosensors-for-on-site}
}