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Makale Tam Makale Uluslararası 2020

Magnetic molecularly imprinted specific solid-phase extraction for determination of dihydroquercetin from Larix griffithiana using HPLC

Xingbin Ma, Xukun Zhang, Hongling Lin, Abdelaty Mostafa Abdelaty Hassıbelnaby, Tsdan Rabah, Xiaoxi Liu, Zhichao Yu, Yanhong Yong, Xianghong Ju, She Yongxin

JOURNAL OF SEPARATION SCIENCE Cilt 43 · Sayı 12 · ss. 2301–2310 · 01.06.2020
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Özet

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimThe naturally occurring quercetin flavonoid, dihydroquercetin, is widely distributed in plant tissues and has a variety of biological activities. Herein, a magnetic molecularly imprinted solid-phase extraction was tailor made for selective determination of dihydroquercetin in Larix griffithiana using high-performance liquid chromatography. Amino-functionalized core-shell magnetic nanoparticles were prepared and characterized using scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, and infrared spectroscopy. The polymer had an average diameter of 250 ± 2.56 nm and exhibited good stability and adsorption for template molecule, which is enriched by hydrogen bonding interaction. Multiple factors for extraction, including loading, washing, elution solvents, and extraction time, were optimized. The limit of detection was 1.23 μg/g. The precision determined at various concentration of dihydroquercetin was less than 4% and the mean recovery was between 74.64 and 101.80%. It has therefore been shown that this protocol can be used as an alternative extraction to quantify dihydroquercetin in L. griffithiana and purify quercetin flavonoid from other complex matrices.

Anahtar kelimeler

SAMPLES ACID POLYMER CHROMATOGRAPHY MICROSPHERES DIHYDROQUERCETIN Molecularly imprinted polymers SILICA SEPARATION RECOGNITION magnetic separation Larix griffithiana Tibetan medicine SELECTIVE EXTRACTION

Atıf için künye

Ma, X., Zhang, X., Lin, H., Hassıbelnaby, A. M. A., Rabah, T., Liu, X., Yu, Z., Yong, Y., Ju, X., & Yongxin, S. (2020). Magnetic molecularly imprinted specific solid-phase extraction for determination of dihydroquercetin from Larix griffithiana using HPLC. JOURNAL OF SEPARATION SCIENCE, 43(12), 2301–2310. https://doi.org/10.1002/jssc.201901086

BibTeX 
@article{ma2020-209649,
  author = {Ma, Xingbin and Zhang, Xukun and Lin, Hongling and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Rabah, Tsdan and Liu, Xiaoxi and Yu, Zhichao and Yong, Yanhong and Ju, Xianghong and Yongxin, She},
  title = {Magnetic molecularly imprinted specific solid-phase extraction for determination of dihydroquercetin from Larix griffithiana using HPLC},
  journal = {JOURNAL OF SEPARATION SCIENCE},
  year = {2020},
  volume = {43},
  number = {12},
  pages = {2301--2310},
  issn = {1615-9306},
  doi = {10.1002/jssc.201901086},
  url = {https://akademik.atauni.edu.tr/yayinlar/209649-magnetic-molecularly-imprinted-specific-solid-phase-extraction-for-determination}
}