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Makale Tam Makale Uluslararası 2022

A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly

Yuanshang Wang, Abdelaty Mostafa Abdelaty Hassıbelnaby, Ge Chen, Huiyan Jia, Xueyan Cui, Lingyuan Xu, Zhen Cao, Yongxin She, Fen Jin, Yudan Zhang, Ahmet Hacımüftüoğlu, Sangqiong Lamu, Jing Wang, LuFei Zheng, Maojun Jin, Bruce D. Hammock

Microchimica Acta Cilt 189 · Sayı 3 · 01.03.2022
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Özet

A rapid detection method is introduced for residual trace levels of triazophos in water and agricultural products using an immunoassay based on catalytic hairpin self-assembly (CHA). The gold nanoparticle (AuNPs) surface was modified with triazophos antibody and sulfhydryl bio-barcode, and an immune competition reaction system was established between triazophos and its ovalbumin-hapten (OVA-hapten). The bio-barcode served as a catalyst to continuously induce the CHA reaction to achieve the dual signal amplification. The method does not rely on the participation of enzymes, and the addition of fluorescent materials in the last step avoids interfering factors, such as a fluorescence burst. The emitted fluorescence was detected at 489/521 nm excitation/emission wavelengths. The detection range of the developed method was 0.01-50 ng/mL for triazophos, and the limit of detection (LOD) was 0.0048 ng/mL. The developed method correlates well with the results obtained by LC-MS/MS, with satisfactory recovery and sensitivity. In sum, the designed method is reliable and provides a new approach to detect pesticide residues rapidly and quantitatively.

Anahtar kelimeler

ASSAY ELECTROCHEMICAL DETECTION FLUORESCENCE DETECTION gold nanoparticles ORGANOPHOSPHORUS PESTICIDES Bio-barcode Triazophos Catalyzed hairpin self -assembly Enzyme-free amplification ULTRASENSITIVE DETECTION

Atıf için künye

Wang, Y., Hassıbelnaby, A. M. A., Chen, G., Jia, H., Cui, X., Xu, L., Cao, Z., She, Y., Jin, F., Zhang, Y., Hacımüftüoğlu, A., Lamu, S., Wang, J., Zheng, L., Jin, M., & Hammock, B. D. (2022). A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly. Microchimica Acta, 189(3). https://doi.org/10.1007/s00604-022-05217-5

BibTeX 
@article{wang2022-209645,
  author = {Wang, Yuanshang and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Chen, Ge and Jia, Huiyan and Cui, Xueyan and Xu, Lingyuan and Cao, Zhen and She, Yongxin and Jin, Fen and Zhang, Yudan and Hacımüftüoğlu, Ahmet and Lamu, Sangqiong and Wang, Jing and Zheng, LuFei and Jin, Maojun and Hammock, Bruce D.},
  title = {A competitive immunoassay for detecting triazophos based on fluorescent catalytic hairpin self-assembly},
  journal = {Microchimica Acta},
  year = {2022},
  volume = {189},
  number = {3},
  issn = {0026-3672},
  doi = {10.1007/s00604-022-05217-5},
  url = {https://akademik.atauni.edu.tr/yayinlar/209645-a-competitive-immunoassay-for-detecting-triazophos-based-on-fluorescent}
}