Chitosan-gentamicin conjugate attenuates heat stress-induced intestinal barrier injury via the TLR4/STAT6/MYLK signaling pathway: In vitro and in vivo studies
Özet
Heat stress (HS) has a negative impact on animal health. A modified chitosan-gentamicin conjugate (CS-GT) was prepared to investigate its potential protective effects and mechanism of action on heat stress-induced intestinal mucosa injury in IPEC-J2 cells and mouse 3D intestinal organs in a mouse model. CS-GT significantly (P < 0.01) reversed the decline in transmembrane resistance and increased the FITC-dextran permeability of the IPEC-J2 monolayer fusion epithelium caused by heat stress. Heat stress decreased the expression of the tight binding proteins occludin, claudin1, and claudin2. However, pretreatment with CS-GT significantly increased (P < 0.01) the expression of these tight binding proteins. Mechanistically, CS-GT inhibited the activation of the TLR4/STAT6/MYLK signaling pathway induced by heat stress. Molecular docking showed that CS-GT can bind effectively with TLR4. In conclusion, CS-GT alleviates heat stress-induced intestinal mucosal damage both in vitro and in vivo. This effect is mediated, at least partly, by the inhibition of the TLR4/STAT6/MYLK signaling pathway and upregulation of tight junction proteins. These findings suggest that CS-GT may have therapeutic potential in the prevention and treatment of heat stress-related intestinal injury.
Anahtar kelimeler
Atıf için künye
Niu, X., Hu, C., Chen, S., Wen, J., Liu, X., Yong, Y., Yu, Z., Ma, X., Li, C., Warda, M. A. A., Hassıbelnaby, A. M. A., Gooneratne, R., & Ju, X. (2023). Chitosan-gentamicin conjugate attenuates heat stress-induced intestinal barrier injury via the TLR4/STAT6/MYLK signaling pathway: In vitro and in vivo studies. CARBOHYDRATE POLYMERS, 321. https://doi.org/10.1016/j.carbpol.2023.121279
BibTeX
@article{niu2023-209589,
author = {Niu, Xueting and Hu, Canying and Chen, Shengwei and Wen, Jiaying and Liu, Xiaoxi and Yong, Yanhong and Yu, Zhichao and Ma, Xingbin and Li, Chengpeng and Warda, Mohamad Alı Ahmad and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Gooneratne, Ravi and Ju, Xianghong},
title = {Chitosan-gentamicin conjugate attenuates heat stress-induced intestinal barrier injury via the TLR4/STAT6/MYLK signaling pathway: In vitro and in vivo studies},
journal = {CARBOHYDRATE POLYMERS},
year = {2023},
volume = {321},
issn = {0144-8617},
doi = {10.1016/j.carbpol.2023.121279},
url = {https://akademik.atauni.edu.tr/yayinlar/209589-chitosan-gentamicin-conjugate-attenuates-heat-stress-induced-intestinal-barrier}
}