Carbamazepine and levetiracetam-loaded PLGA nanoparticles prepared by nanoprecipitation method:in vitroandin vivostudies

Kandilli, B; Uğur Kaplan, Afife Büşra; Çetin, Meltem; Taspinar, N; Ertugrul, MS; Aydin, İsmail Çağrı; Hacımüftüoğlu, Ahmet

doi:10.1080/03639045.2020.1769127

Makale Tam Makale Uluslararası 2020

Carbamazepine and levetiracetam-loaded PLGA nanoparticles prepared by nanoprecipitation method:in vitroandin vivostudies

B Kandilli, Afife Büşra Uğur Kaplan, Meltem Çetin, N Taspinar, MS Ertugrul, İsmail Çağrı Aydin, Ahmet Hacımüftüoğlu

DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY Cilt 46 · Sayı 7 · ss. 1063–1072 · 01.07.2020

DOI ile aç

Özet

Objective:The aim of this study was to develop the PLGA nanoparticles (NPs) containing carbamazepine (CBZ) and levetiracetam (LEV) combination (CBZ + LEV) for the treatment of epilepsy and toin vitrocharacterize the prepared NPs. Significance:LEV and CBZ, which are antiepileptic drugs, are used in the treatment of epilepsy. Nano-sized formulations are prepared to use for different purposes such as to improve the solubility/the physicochemical properties and bioavailability of drugs, to decrease their doses and frequency of administration, and to reduce side effects of drugs. Methods:CBZ + LEV-PLGA-NPs were prepared by a modified nanoprecipitation method andin vitroandin vivocharacterized. Also, the antiepileptic effect of the NPs was evaluatedin vivoin a rat epilepsy model. Results:The mean particle size and zeta potential of CBZ + LEV-PLGA-NPs were 180.62 +/- 6.260 nm and -27.08 +/- 3.110 mV, respectively. The values of encapsulation efficiency (EE%) of CBZ and LEV were 51.00 +/- 5.944% and 2.05 +/- 0.367%, respectively. CBZ showed a biphasic release profile with initial burst release followed by a sustained release. Ninety percent of CBZ was released from NPs within two days; however, % LEV release from NPs reached about 80% within 30 min. Conclusion:Our results showed that a decrease in seizure scores in the group treated with CBZ + LEV was observed and also, CBZ + LEV and CBZ + LEV-PLGA-NPs had similar antiepileptic activity. The NPs containing CBZ + LEV might be beneficial in the treatment of epilepsy.

Anahtar kelimeler

nanoparticles PLGA Carbamazepine epilepsy levetiracetam

Atıf için künye

Kandilli, B., Uğur Kaplan, A. B., Çetin, M., Taspinar, N., Ertugrul, M., Aydin, İ. Ç., & Hacımüftüoğlu, A. (2020). Carbamazepine and levetiracetam-loaded PLGA nanoparticles prepared by nanoprecipitation method:in vitroandin vivostudies. DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 46(7), 1063–1072. https://doi.org/10.1080/03639045.2020.1769127

BibTeX

@article{kandilli2020-20667,
  author = {Kandilli, B and Uğur Kaplan, Afife Büşra and Çetin, Meltem and Taspinar, N and Ertugrul, MS and Aydin, İsmail Çağrı and Hacımüftüoğlu, Ahmet},
  title = {Carbamazepine and levetiracetam-loaded PLGA nanoparticles prepared by nanoprecipitation method:in vitroandin vivostudies},
  journal = {DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY},
  year = {2020},
  volume = {46},
  number = {7},
  pages = {1063--1072},
  issn = {0363-9045},
  doi = {10.1080/03639045.2020.1769127},
  url = {https://akademik.atauni.edu.tr/yayinlar/20667-carbamazepine-and-levetiracetam-loaded-plga-nanoparticles-prepared-by}
}