Detection report of a therapeutic effect of Linum usitatissimum oil against glyphosate toxicity on a fish modelling using a multiple comparison biology-based approach: Hematological alterations, oxidative stress and cytokine enzyme biomarkers, DNA damage, cellular apoptosis, and gene expression
Özet
In this study, the therapeutic effect of linseed-oil (LO) on pesticide toxicity in aquatic environment was investigated for the first time. It was aimed to determine the possible therapeutic effect of LO-enriched diets on the target tissues (blood, gill and liver) of rainbow trout (Oncorhynchus mykiss, initial weight: 32 ± 2 g) against sub-chronic glyphosate toxicity. Fish sub-chronically exposed to 5 mg/kg glyphosate (GF) were simultaneously fed 8 different experimental diets (Control, Low- Linseed-oil (L-LO), Medium- Linseed-oil (M-LO), High- Linseed-oil (H-LO), Low- Linseed-oil + glyphosate (L-LO+GF), Medium- Linseed-oil + glyphosate (M-LO+GF), High- Linseed-oil + glyphosate (H-LO+GF) and glyphosate (GF)) enriched with different levels of LO (0%, 12.5 mL/kg (Low), 25 mL/kg (Medium) and 37.5 mL/kg (High)). At the end of the feed ing period, in addition to the determination of biomass data, hematological indices in blood tissue; hematological indices, oxidative stress response (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), reactive oxygen species (ROS), malondialdehyde (MDA levels), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), Nuclear factor erythroid 2 level (Nrf-2) in liver and gill tissue, apoptosis (Caspas3) activities and DNA damage (8-OHdG) and molecular-based cytochrome P450 (CYP450 (Stp450)), heat shock protein (HSP70) and apoptosis marker (Caspas3 expressions) were monitored as a mechanism of therapeutic effect of LO accompanying GF toxicity and its effectiveness as a possible treatment modality. Our findings confirmed that glyphosate-mediated toxicity in O. mykiss produced adverse effects such as growth suppression, oxidative stress and hematopoietic impairment, and that flaxseed oil as a therapeutic agent can be used as a sustainable biological protective dietary supplement.
Anahtar kelimeler
Atıf için künye
Alak, G., Taşçı, E. R., Yeltekin, A. Ç., Önalan, Ş., Özçelik, E. Z., Koşar, K., Ekiz, E. E., Yaman, A., Ekiz, M. A., Akbey, T., Uçar, A., Aydağ, B., Kaya, E., Parlak, V., & Atamanalp, M. (2026). Detection report of a therapeutic effect of Linum usitatissimum oil against glyphosate toxicity on a fish modelling using a multiple comparison biology-based approach: Hematological alterations, oxidative stress and cytokine enzyme biomarkers, DNA damage, cellular apoptosis, and gene expression. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 125. https://doi.org/10.1016/j.etap.2026.105091
BibTeX
@article{alak2026-205421,
author = {Alak, Gonca and Taşçı, Ebrar Rana and Yeltekin, Aslı Çilingir and Önalan, Şükrü and Özçelik, Elif Zeynep and Koşar, Koray and Ekiz, Eylül Ecrin and Yaman, Azra and Ekiz, Muhammed Alaeddin and Akbey, Tuba and Uçar, Arzu and Aydağ, Burak and Kaya, Emircan and Parlak, Veysel and Atamanalp, Muhammed},
title = {Detection report of a therapeutic effect of Linum usitatissimum oil against glyphosate toxicity on a fish modelling using a multiple comparison biology-based approach: Hematological alterations, oxidative stress and cytokine enzyme biomarkers, DNA damage, cellular apoptosis, and gene expression},
journal = {ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY},
year = {2026},
volume = {125},
issn = {1382-6689},
doi = {10.1016/j.etap.2026.105091},
url = {https://akademik.atauni.edu.tr/yayinlar/205421-detection-report-of-a-therapeutic-effect-of-linum-usitatissimum-oil-against}
}