Effects of different concentrations of diazinon on 8-hydroxy-2-deoxyguanosine and histopathology, antioxidant enzyme, acetylcholinesterase activity and plasma metabolites in rainbow trout (Oncorhynchus mykiss)

The aim of this study was to determine the all of effects on the liver of diazinon. Here, we were to investigation the effects of different concentrations of diazinon on 8-hydroxy-2-deoxyguanosine (8-OHdG) and histopathology, antioxidant enzymes, the plasma metabolites of rainbow trout (Onchorhynchus mykiss). During the six days, 15 rainbow trout were used for each group and then were subjected to two different concentration (0.15 mg/L and 0.30 mg/L) of Diazinon, respectively (LC50=1.65 mg/L). A 0.15 mg/L level of diazinon did not effect aspartate aminotransferase (AST) and alanine aminotransferase (ALT). But, 0.30 mg/L concentration caused a significant increase in both parameters. 0.15 mg/L of diazinon caused a significant decrease in the lactate dehydrogenase (LDH) level. 0.15 and 0.30 mg/L of diazinon significantly increased antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR)). But, the total antioxidant capacity (TA) capacity, acetylcholinesterase activity (AChE), albumin (AL) and total protein (TP) levels significantly decreased at both concentration levels when compared to the control group (P < 0.05). There were significant changes in 8-OHdG levels and histopathological damage in liver tissues. As a results; diazinon caused changes in the histopathological and 8-OHdG and antioxidant enzymes as well as plasma AChE activity and metabolites of rainbow trout. (C) 2019 Friends Science Publishers