The effects of imidacloprid on 8-hydroxy-2-eoxyguanosine (8-OHdG) and antioxidant parameters in gill and liver tissues of trout

In this research, it was aimed to determine the effects of imidacloprid on 8-hydroxy-2-deoxyguanosine (8-ohdg) and antioxidant parameters in the liver and gill tissues of rainbow trout (Oncorhynchus mykiss). Three different concentrations (5 mg/L, 10 mg/ L and 20 mg/L) of the imidacloprid were routinely exposed to rainbow trout for 21 days. Antioxidant enzymes, malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHDG) were studied in the samples. In the 5 mg/L experimental group, it was determined that CAT values and liver GPx and SOD levels increased compared to those in the control group. It was found that there were significant increases between 5 mg/L and 10 mg/L dose groups of MDA, 8-OHdG, liver GPx-SOD and gill CAT-GPx enzyme activities (p < 0.05). High increases in all antioxidant enzyme levels were observed in liver and gill tissues of fish exposed to 20 mg/L dose imidacloprid. 8-OHdG and MDA levels significantly increased in parallel with the increase in the dose (p <0.05). These results suggested that imidacloprid can be toxic to fish by leading to an increase in 8-OHdG activity and changes in oxidative stress parameters. Therefore, these data may reflect one of the molecular pathway that play roles in imidacloprid toxicity.