Antibacterial Effect of Hypericum Perforatum and Calophyllum Inophyllum Against Some Bacteria Causing Infections in Cesarean and Episiotomy Wounds

Purpose: This study was carried out to examine the antibacterial effect of Hypericum perforatum essential oil

and methanol extract and Calophyllum inophyllum seed oil against some bacteria that lead to recovery and

infections in cesarean and episiotomy wounds.

Methods/Design: Bacteria isolated from bacterial cultures of cesarean and episiotomy incisions were identified

using the conventional Vitek diagnostic method, automatized for validation, which is used in routine

microbiology. Hypericum perforatum methanol extract, pure Calophyllum inophyllum seed oil, Hypericum

perforatum essential oil prepared in Calophyllum inophyllum seed oil were used. The “agar disk diffusion”

method, which is the most widely used and recommended method for antibacterial activity in natural plant

extracts for the imaging of antibacterial activity, the microdilution method, also known as “Micro-well plate

dilution” and using 96-well U-bottom microplates, were used. When two methods are evaluated in terms of

sensitivity in the study, considering our disk diffusion test results, microorganisms obtained from the routine

microbiology wound culture isolates have a high antibacterial effect due to C. inophyllum.

Results: The experiment was carried out with the microwell dilution method with regard to sensitivity, in which

the first dilutions were initiated from ½ dilution, and a total of 8 dilutions were studied. The results of this

experiment were particularly pleasant for C.inophyllum on Staphylococcus species, and the MIC values

determined for 12 isolates from the corresponding extracts are presented.

Conclusion: As a result of the analyses, it was found out that Calophyllum inophyllum was more effective on

bacteria than Hypericum perforatum.