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Makale Tam Makale Uluslararası 2022

Hematotoxic, oxidative and genotoxic damage in rainbow trout (Oncorhynchus mykiss) after exposure to 3-benzoylpyridine

Veysel Parlak, Bunyamin Ozgeris, Arzu Uçar, Aslı Çilingir Yeltekin, Fatma Betül Özgeriş, Ozge Caglar, Gonca Alak, Hasan Türkez, Muhammed Atamanalp

TOXICOLOGY MECHANISMS AND METHODS Cilt 32 · Sayı 7 · ss. 501–509 · 01.09.2022
DOI ile aç

Özet

Pyridine is a basic heterocyclic organic compound. The pyridine ring is present in many important compounds, including agricultural chemicals, medicines and vitamins. Due to their widespread industrial use, bioaccumulation and non-target toxic effects are being considered as a great risk to human and environmental health. In this study, we aimed to evaluate the hematological, oxidative and genotoxic damage potentials by different concentrations (1, 1.5, and 2 g/L) of the ketone 3-Benzoylpyridine (3BP) on rainbow trout (Oncorhynchus mykiss). Alterations in the biomarker levels of oxidative DNA damage (8-hydroxy-2 '-deoxyguanosine (8-OHdG)), apoptosis (Caspase-3), malondialdehyde (MDA) as well as antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), myeloperoxidase (MPO), paraoxonase (PON), and arylesterase (AR) were assessed in brain, liver, gill and blood tissues. Acetylcholinesterase (AChE) activity was also determined in brain tissue. In addition, we analyzed micronucleus (MN) rates and hematological indices of total erythrocyte count (RBC), total leukocyte count (WBC), hemoglobin (Hb), hematocrit (Hct), total platelet count (PLT), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), and mean cell volume (MCV) in blood. LC50-96h value of 3BP was calculated as 5.2 g/L from the data obtained. A significant decrease in brain AChE activity was determined in clear time and dose dependent manners. While SOD, CAT, GPx, PON, and AR levels were decreased, MDA, MPO, 8-OHdG and Caspase-3 levels were increased in all tissues (p < 0.05). Again, the 3BP led to increases of MN formation at all applied concentrations in the rates of between 45.4 and 72.7%. Significant differences (p < 0.05) were found out in between all studied hematology parameters between 3BP-exposed and the control fish. In conclusion, ours study firstly indicated that the treatment doses of 3BP induced distinct hematological and oxidative alterations as well as genotoxic damage in rainbow trout.

Anahtar kelimeler

stress oxidative stress PERFORMANCE LIPID-PEROXIDATION antioxidant GENE-EXPRESSION PARAMETERS DNA-DAMAGE hepatotoxicity NEUROTOXICITY Rainbow trout RESPONSES micronucleus DANIO-RERIO PESTICIDE hematotoxicity CARASSIUS-AURATUS 3-Benzoylpyridine

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

Parlak, V., Ozgeris, B., Uçar, A., Yeltekin, A. Ç., Özgeriş, F. B., Caglar, O., Alak, G., Türkez, H., & Atamanalp, M. (2022). Hematotoxic, oxidative and genotoxic damage in rainbow trout (Oncorhynchus mykiss) after exposure to 3-benzoylpyridine. TOXICOLOGY MECHANISMS AND METHODS, 32(7), 501–509. https://doi.org/10.1080/15376516.2022.2049413

BibTeX 
@article{parlak2022-200816,
  author = {Parlak, Veysel and Ozgeris, Bunyamin and Uçar, Arzu and Yeltekin, Aslı Çilingir and Özgeriş, Fatma Betül and Caglar, Ozge and Alak, Gonca and Türkez, Hasan and Atamanalp, Muhammed},
  title = {Hematotoxic, oxidative and genotoxic damage in rainbow trout (Oncorhynchus mykiss) after exposure to 3-benzoylpyridine},
  journal = {TOXICOLOGY MECHANISMS AND METHODS},
  year = {2022},
  volume = {32},
  number = {7},
  pages = {501--509},
  issn = {1537-6516},
  doi = {10.1080/15376516.2022.2049413},
  url = {https://akademik.atauni.edu.tr/yayinlar/200816-hematotoxic-oxidative-and-genotoxic-damage-in-rainbow-trout-oncorhynchus-mykiss}
}