Effect of Different Extraction Media on Quantification of the Released Monomers from Dental Composite

May factors affect the amount of released monomers from composite resins. The aim of this study is to measure the release of monomers from microhybrid composite resin Filtek Z250 (3 M ESPE, USA) for a long time [1 h, 24 h, 7 d (168 h) and 30 d (720 h)] after the polymerization and observe the effect of different extraction media [ethanol/water solutions (75:25, v/v), distilled water, artificial saliva] on released monomers. Twelve specimen discs were prepared using composite resin for each analyte. Quantitative analysis of released monomers from composite resin was determined by using high performance liquid chromatography. Data were analyzed using one way ANOVA and Duncan test. All monomers were released in ethanol/water solution higher efficiency than the other media and the highest amount of release is obtained from BisGMA (p < 0.5). Maximum release of all monomers occurred within the first hour and after 24 h, the amount of released monomer generally reduced by 30 d.