Integrating biotechnology into diagnostic labs: Innovations in PCR and molecular procedures
Tahir, Muhammad Faran, Ishtiaq, Sidra, Anas, Muhammad, Irfan, Ayesha, Jaremko, Mariusz, Fahad, Shah, Ercisli, Sezai, Almahasheer, Hanan, Abdelsalam, Nader R.
The field of biotechnology has significantly contributed to progress in diagnostic laboratories, especially in the early identification of genetic disorders. Timely detection plays a vital role in the treatment and management of such conditions, and numerous molecular approaches have been established to identify disease markers with high accuracy and sensitivity. The polymerase chain reaction (PCR), a cornerstone technique in molecular biology, has seen various advancements, establishing it as an essential method in diagnostic settings for recognizing genetic abnormalities. PCR-based approaches have transformed the diagnostic process by amplifying trace amounts of DNA, allowing for the identification of mutations linked to a wide range of inherited conditions. This review explores the role of biotechnology in diagnostic labs, emphasizing genetic disease detection through molecular procedures. Special attention is given to innovations in PCR, highlighting new PCR-based techniques such as digital PCR, multiplex PCR, and reverse-transcription PCR, which have improved sensitivity and versatility. Additionally, various PCR methodologies, their diagnostic applications, and inherent limitations are discussed. The role of molecular tools in identifying diseases such as cancer, hereditary disorders, and infectious infections is also explored. The review further outlines the evolution of PCR technologies, their integration into molecular diagnostics, and their potential to improve diagnostic accuracy and enable early disease detection. Ongoing advancements in PCR technologies are transforming diagnostic practices by enabling more accurate, faster, and widely accessible molecular testing. These innovations promise enhanced disease management and better clinical outcomes, heralding a new era in healthcare.
- Journal
- CURRENT RESEARCH IN BIOTECHNOLOGY
- Year
- 2025
- Volume
- 10
- ISSN
- 2590-2628
- DOI
- 10.1016/j.crbiot.2025.100325
- WoS UID
- WOS:001565784600001