Innovative orthogonal two-dimensional reversed-phase liquid chromatography x supercritical fluid chromatography with a phenyl/ tetrazole stationary phase for the preparative isolation of diarylheptanoids
Dang, Jun, Tong, Yingying, Wang, Qilan, Li, Gang, Abd El-Aty, A. M.
In this investigation, we successfully isolated and purified natural diarylheptanoids using an orthogonal offline two-dimensional RPLC x SFC approach, employing only the phenyl/tetrazole stationary phase. First, a styrenedivinylbenzene matrix medium pretreatment liquid chromatography system effectively processed chlorophyllcontaining plant extract solution with a recovery rate of 33.8 %, obviating the need for concentration steps. Subsequently, an offline two-dimensional RPLC x SFC employing only the phenyl/tetrazole stationary phase achieved a remarkable 96.38 % orthogonality and was established and utilized in the preparative separation and purification of natural products. Finally, the constructed single stationary phase highly orthogonal RPLC x SFC system was successfully applied in the preparative separation and purification of natural diarylheptanoids from the Saxifraga tangutica target fraction and yielded four diarylheptanoids with purities exceeding 95 %.
- Journal
- JOURNAL OF CHROMATOGRAPHY A
- Year
- 2024
- Volume
- 1726
- ISSN
- 0021-9673
- eISSN
- 1873-3778
- DOI
- 10.1016/j.chroma.2024.464950
- WoS UID
- WOS:001238035900001