Article 2023 · 4 citations

Plant growth regulators ameliorate biochemical and molecular parameters in Plantago ovata Forssk. under salt stress

Samad, Abdul, Shaukat, Kanval, rahman, Mahmood Ur, Ahmad, Hafiz Muhammad, Nizar, Mereen, Hakeem, Abdul, Fiaz, Sajid, Alafari, Hayat Ali, Jalal, Areej S., Attia, Kotb A., Karatas, Neva

Abstract

Salinity is a major environmental stress affecting the growth and development of plants. Saline soils limit crop productivity, resulting in economic losses by compromising morpho-physiological and yield attributes of plants. However, the exogenous application of different plant growth regulators (PGRs) helps to ameliorate the negative effects of salinity. A study was carried out to explore the impact of foliar applications of different PGRs i.e. Salicylic Acid (SA) 250 mu M, Thiourea (TU) 10mM, dry Moringa Leaf Extract (MLE) 10% and Proline 1 mu M to mitigate salt stress (NaCl 120mM) in medicinally important plant, Plantago ovata. Salinity hampered shoot length, root and shoot dry matter, fresh weight as well as number of leaves. However, foliar application of PGRs helped to improve various growth attributes like total number of leaves per plant, root length, shoot length, root fresh weight, root dry weight, shoot fresh weight, and shoot dry weight. Moreover, biochemicals i.e. hydrogen peroxide (H2O2) and malondialdehyde (MDA) were substantially increased under salt stress indicating increased reactive oxygen species (ROS). In response to ROS, antioxidants were found to be upregulated when the expression of antioxidant genes like catalase (CAT), Glutathione-S-transferase (GST), and Glutathione Reductase (GR) were analyzed under salinity stress. However, the expression of GR, CAT, and GST was further enhanced after the application of PGRs. Their expression was many folds higher (GR up to 7.4, CAT up to 9.5, and GST up to 7.9 folds higher) in salt-subjected plants as compared to control, suggesting their role in salt stress tolerance. It is concluded that foliar application of PGRs particularly TU and MLE improved morphological parameters and may be recommended for improved growth of plants under salinity stress.

Keywords

Enzymatic antioxidants reactive oxygen species gene expression sustainable agriculture OXIDATIVE STRESS PROMOTING RHIZOBACTERIA EXOGENOUS APPLICATION FOLIAR APPLICATION MORINGA-OLEIFERA SALINITY STRESS NUTRIENT-UPTAKE SALICYLIC-ACID NITRIC-OXIDE L.

WoS Categories

Agriculture, Multidisciplinary Agronomy Forestry

Sustainable Development Goals

Zero Hunger Climate Action

Citation

Journal: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
Year: 2023
Volume: 47
Issue: 5
Pages: 676-687
ISSN: 1300-011X
eISSN: 1303-6173
DOI: 10.55730/1300-011X.3120
WoS UID: WOS:001087372800008

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