Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables

Imidacloprid is one of the most commonly used insecticides for managing pests, thus, improving the quality and yield of vegetables. The abuse/misuse of imidacloprid contaminates the environment and threatens human health. To reduce the risk, a colorimetric enzyme-linked immunoassay assay (Co-ELISA) and chemiluminescence enzyme-linked immunoassay assay (Cl-ELISA) were established to detect imidacloprid residues in vegetables. The linear range of Co-ELISA ranged between 1.56 mu g/L and 200 mu g/L with a limit of detection (LOD) of 1.56 mu g/L. The values for Cl-ELISA were 0.19 mu g/L to 25 mu g/L with an LOD of 0.19 mu g/L, which are lower than those of Co-ELISA. Fortifying Chinese cabbage, cucumber, and zucchini with imidacloprid at 10, 50, and 100 mu g/L yielded recoveries between 81.7 and 117.6% for Co-ELISA and at 5, 10, and 20 mu g/L yielded recoveries range from 69.7 to 120.6% for Cl-ELISA. These results indicate that Cl-ELISA has a high sensitivity and a rapid detection time, saving cost (antigen and antibody concentrations) and serving as a more efficient model for the rapid detection of imidacloprid residue.

Journal

FOODS

Year

2023

Volume

12

Issue

1

eISSN

2304-8158

DOI

10.3390/foods12010196

WoS UID

WOS:000909873700001