Article 2022 · 8 citations

Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach

Razgonova, Mayya P. P., Zinchenko, Yulia N. N., Kozak, Darya K. K., Kuznetsova, Victoria A. A., Zakharenko, Alexander M. M., Ercisli, Sezai, Golokhvast, Kirill S. S.

Abstract

In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.

Keywords

Glycine Willd flavonols laser microscopy HPLC-MS MS tandem mass spectrometry polyphenols COWPEA VIGNA-UNGUICULATA HPLC-DAD ANTIOXIDANT ACTIVITY SECONDARY METABOLITES CELL-WALLS SEED COATS IDENTIFICATION L. QUANTIFICATION POLYPHENOLS

WoS Categories

Biochemistry & Molecular Biology Chemistry, Multidisciplinary

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: MOLECULES
Year: 2022
Volume: 27
Issue: 23
eISSN: 1420-3049
DOI: 10.3390/molecules27238228
WoS UID: WOS:000897373000001

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