Article 2022 · 6 citations

Recovering triploid citrus hybrids from 2x x 2x sexual crosses with the aid of embryo rescue and flow cytometry in Turkey

Cimen, Berken, Yesiloglu, Turgut, Donmez, Dicle, Kacar, Yildiz Aka, Ercisli, Sezai

Abstract

Background Turkey is one of the major exporters of mandarins in the Mediterranean region. Seedlessness in citrus, which is one of the most desired fruit quality traits, especially in fresh mandarin export markets, can be obtained via triploidy as in many fruit species. Triploid plants can be recovered by 2x x 2x hybridizations in citrus, as well as 2x x 4x and 4x x 2x crosses. Hence, we aimed to develop local triploid hybrids by using the embryo rescue technique in five crosses using eight different citrus varieties in the present study. Methods and results Embryos isolated from abortive seeds derived by 135 days after pollinations were cultured on modified Murashige and Tucker (MT) medium by adding different levels of GA(3) to achieve a high germination rate. A population of 574 plants was developed as a result of embryo rescue. After screening the ploidy levels of this 574-plant population with the aid of flow cytometry, 4 triploids from 'Encore' x 'Murcott', 8 triploids from 'Fortune' x 'Willow leaf', 1 triploid from 'Kiyomi' x 'Murcott', and 1 triploid from 'Ortanique' x 'Murcott' hybridization were recovered. Triploid hybrid plants and related parents were analyzed with SSR markers heterozygotic for parental mandarin varieties. In addition, we evaluated stomatal characteristics of diploid and triploid hybrids obtained from different crosses. Stomatal traits of diploid and triploid hybrids in all crosses significantly differed except the stomata index. Conclusions Genotyping of triploid plants confirmed using Simple Sequence Repeat (SSR) molecular markers and five SSRs were able to identify three alleles of triploid hybrids. Selected triploid mandarin hybrids have been grafted on several rootstocks for field trials and are in the process of yield and quality performances.

Keywords

Polyploidy Embryo rescue Plant breeding Mandarin Flow cytometry SSR SOMATIC EMBRYOGENESIS NUCELLAR SEEDLINGS SEED DEVELOPMENT IDENTIFICATION PLOIDY POLYEMBRYONY POLYPLOIDS INDUCTION ORIGIN ORANGE

WoS Categories

Biochemistry & Molecular Biology

Sustainable Development Goals

Life on Land

Citation

Journal: MOLECULAR BIOLOGY REPORTS
Year: 2022
Volume: 49
Issue: 6
Pages: 5625-5634
ISSN: 0301-4851
eISSN: 1573-4978
DOI: 10.1007/s11033-022-07555-2
WoS UID: WOS:000803774900001

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