Article 2021 · 7 citations

Simple Sequence Repeat and S-Locus Genotyping to Assist the Genetic Characterization and Breeding of Polyploid Prunus Species, P. spinosa and P. domestica subsp. insititia

Halasz, Julia, Makovics-Zsohar, Noemi, Szoke, Ferenc, Ercisli, Sezai, Hegedus, Attila

Abstract

Polyploid Prunus spinosa (2n = 4 x) and P. domestica subsp. insititia (2n = 6 x) represent enormous genetic potential in Central Europe, which can be exploited in breeding programs. In Hungary, 16 cultivar candidates and a recognized cultivar 'Zempleni' were selected from wild-growing populations including ten P. spinosa, four P. domestica subsp. insititia and three P. spinosa x P. domestica hybrids (2n = 5 x) were also created. Genotyping in eleven simple sequence repeat (SSR) loci and the multiallelic S-locus was used to characterize genetic variability and achieve a reliable identification of tested accessions. Nine SSR loci proved to be polymorphic and eight of those were highly informative (PIC values > 0.7). A total of 129 SSR alleles were identified, which means 14.3 average allele number per locus and all accessions but two clones could be discriminated based on unique SSR fingerprints. A total of 23 S-RNase alleles were identified and the complete and partial S-genotype was determined for 10 and 7 accessions, respectively. The DNA sequence was determined for a total of 17 fragments representing 11 S-RNase alleles. 'Zempleni' was confirmed to be self-compatible carrying at least one non-functional S-RNase allele (S-J). Our results indicate that the S-allele pools of wild-growing P. spinosa and P. domestica subsp. insititia are overlapping in Hungary. Phylogenetic and principal component analyses confirmed the high level of diversity and genetic differentiation present within the analysed accessions and indicated putative ancestor-descendant relationships. Our data confirm that S-locus genotyping is suitable for diversity studies in polyploid Prunus species but non-related accessions sharing common S-alleles may distort phylogenetic inferences.

Keywords

Prunus spinosa Prunus domestica subsp insititia SSR Self-incompatibility S-genotype Polyploid GAMETOPHYTIC SELF-INCOMPATIBILITY POSITIVELY SELECTED SITES MICROSATELLITE MARKERS CHLOROPLAST DNA RNASE ALLELES SSR MARKERS PERSICA L. AVIUM L. CHERRY DIVERSITY

WoS Categories

Biochemistry & Molecular Biology Genetics & Heredity

Sustainable Development Goals

Life on Land Climate Action

Citation

Journal: BIOCHEMICAL GENETICS
Year: 2021
Volume: 59
Issue: 4
Pages: 1065-1087
ISSN: 0006-2928
eISSN: 1573-4927
DOI: 10.1007/s10528-021-10090-7
WoS UID: WOS:000662115200001

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