Article 2022 · 16 atıf

Some indazoles as alternative inhibitors for potato polyphenol oxidase

Ozturk, Cansu, Bayrak, Songul, Demir, Yeliz, Aksoy, Mine, Alim, Zuhal, Ozdemir, Hasan, Kufrevioglu, Omer Irfan

Özet

Fresh-cut vegetables and fruits have gained attention among consumers because of their fresh appearance, lack of pollution, nutrition, and convenience. However, in fresh-cut foods, enzymatic browning is the main problem. Polyphenol oxidase (PPO) is a vital enzyme involved in the process of enzymatic browning. In this study, PPO was purified from potato using Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity chromatography and the effect of some indazoles on the enzyme was determined. The enzyme was purified with a specific activity of 52,857.14 EU/mg protein and 21.26-purification fold. Indazoles exhibited inhibitor properties for PPO with IC50 values in the range of 0.11-1.12 mM and K-i values in the range of 0.15 +/- 0.04-3.55 +/- 0.88 mM. Among these compounds, 7-chloro-1H-indazole was shown as the most potent PPO inhibitor (K-i: 0.15 +/- 0.04 mM). Determination of the enzyme's inhibition kinetics will simplify the testing of candidate PPO inhibitors.

Anahtar Kelimeler

affinity chromatography enzyme purification indazoles inhibition polyphenol oxidase IN-SILICO EVALUATION CARBONIC-ANHYDRASE POTENTIAL ACETYLCHOLINESTERASE PARTIAL-PURIFICATION VITRO TYROSINASE L. KINETICS

WoS Kategorileri

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology

Künye

Dergi: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Yıl: 2022
Cilt: 69
Sayı: 5
Sayfa: 2249-2256
ISSN: 0885-4513
eISSN: 1470-8744
DOI: 10.1002/bab.2283
WoS UID: WOS:000723243900001

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