Waste frying oil hydrolysis and lipase production by cold-adapted<i>Pseudomonas yamanorum</i>LP2 under non-sterile culture conditions

Non-sterile culture technique is currently used in some microbial processes. However, there is no study on the use of this technique in the production of microbial lipases and hydrolysis of waste frying oils. This study was conducted to hydrolyse waste frying oils and produce lipase under non-sterile culture conditions using locally isolated cold-adapted bacteria. Of 75 bacterial isolates, the psychrotolerantPseudomonas yamanorumLP2 (Genbank number: KU711080) was determined to have the highest lipase activity. It was found that a combination of restricted nutrient availability, low temperature and high inoculum volume prevented microbial contaminants under non-sterile conditions. The most favourable parameters for lipase production under both sterile and non-sterile conditions were 15 degrees C temperature, pH 8, 30 mL/L inoculum volume, 40 mL/L waste frying oil concentration, 10 mL/L Tween-80 and 72 h incubation time. The maximum lipase activities in sterile and non-sterile media were determined as 93.3 and 96.8 U/L, respectively. The present process designed for enzyme production and waste oil hydrolysis can reduce the cost of cultivation medium as well as energy consumption and workload. The potential of cold-adapted bacteria to produce lipase and hydrolyse waste oils under non-sterile culture conditions was first tested in the current study.

Dergi

ENVIRONMENTAL TECHNOLOGY

Yıl

2021

Cilt

42

Sayı

20

Sayfa

3245-3253

ISSN

0959-3330

eISSN

1479-487X

DOI

10.1080/09593330.2020.1745297

WoS UID

WOS:000557975300001