Genetically Engineered Nanobody-Recombinant Antibody Colloidal Gold Strip for Ultrasensitive Atrazine Detection in Fruits and Vegetables
Bao, Jiayong, Li, Peipei, El-Aty, A. M. Abd, Zhao, Jing, Xu, Lingyuan, Guo, Chenxi, Li, Jia, Lei, Xingmei, Feng, Sensen, Liu, Yi, Chen, Si, Jin, Fen, Wang, Jing, Zheng, Lufei, Cao, Zhen, Hammock, Bruce D., Jin, Maojun
Colloidal gold immunochromatography (CG-ICA) is distinguished by its cost-effectiveness and rapid detection capabilities. However, its key recognition components-antigens and antibodies-are typically produced through chemical synthesis or ascites induction techniques. These methods often lead to batch-to-batch variability and the risk of cell line loss, thereby limiting the production efficiency of antigens and antibodies. In this study, using atrazine as the target analyte, a novel colloidal gold test strip, AId/rAb-ICA (Anti-Idiotypic nanobody/recombinant full-length antibody-immunochromatographic assay), was successfully developed by employing an anti-idiotypic nanobody (AI-Nb-66) and a recombinant full-length antibody (ATR-rAb) to replace the traditional antigen-antibody pair in CG-ICA. The detection limit (LOD) of this test strip was 0.31 ng/mL. It was successfully validated in apples, celery, and scallions, with recovery rates ranging from 82.45% to 103.60% and coefficients of variation (CVs) not exceeding 7.80%. This study offers a new approach for atrazine detection and the development of colloidal gold test strips.
- Journal
- FOOD SAFETY AND HEALTH
- Year
- 2026
- ISSN
- 2835-1096
- DOI
- 10.1002/fsh3.70089
- WoS UID
- WOS:001713361900001