Biological treatment of dye wastewater with black radish peroxidase immobilized on CoMoO₄ nanoflowers

This study presents the immobilization of black radish peroxidase (Br-POD) on CoMoO4 (CMO) nanoflowers and evaluates its performance in the oxidative degradation of methylene blue (MB), a commonly used industrial dye. The immobilization process achieved a high efficiency of 83.87 %, with a specific activity of 5.53 U/mg for the immobilized enzyme. Morphological and structural analyses by FE-SEM and FT-IR were confirmed the successful attachment of Br-POD to the CMO surface without significant conformational alterations. Catalytic performance tests revealed that the immobilized Br-POD exhibited 91.0 % MB decolorization efficiency under optimal conditions (50 degrees C, pH 6.5, 0.6 mM H2O2), outperforming the free enzyme, which achieved only 61.5 % under the same conditions. Notably, Br-POD@CMO retained 91.0 % of its initial activity after seven consecutive reuse cycles, indicating excellent operational stability. The immobilized enzyme also exhibited enhanced resistance against thermal denaturation, maintaining higher activity across a broader pH and temperature range. Furthermore, the Br-POD@CMO composite demonstrated increased tolerance against inhibitory metal ions (e.g., Zn2+, Hg2+), further supporting its potential for industrial wastewater treatment applications. These findings suggest that CMO nanoflowers are a practical support for peroxidase immobilization, offering enhanced stability, reusability, and catalytic performance, and represent a promising strategy for sustainable dye wastewater remediation.

Journal

JOURNAL OF THE INDIAN CHEMICAL SOCIETY

Year

2025

Volume

102

Issue

12

ISSN

0019-4522

DOI

10.1016/j.jics.2025.102196

WoS UID

WOS:001608046900002