Article 2025 · 8 citations

Immobilized Black Radish Peroxidase on ZnO-Alginate Beads: A Multifunctional Biocatalyst for Sustainable Food Packaging

Abstract

This study presents a novel bioactive food packaging material developed by immobilizing black radish peroxidase (Br-POD) calcium alginate beads loaded with zinc oxide nanoparticles (ZnO/CA). The immobilization process achieved an efficiency of 90.6% and increased enzyme-specific activity by 36%. The ZnO/CA-Br-POD composite exhibited enhanced thermal stability (optimal temperature shift from 40 degrees C to 60 degrees C) and pH tolerance (from pH 6 to 5), along with strong antimicrobial activity against Staphylococcus aureus and Escherichia coli. Antioxidant performance, evaluated via DPPH and ABTS radical scavenging assays, showed up to 93.4% activity. Application on Agaricus bisporus mushrooms reduced weight loss by 49.5% during storage, demonstrating significant preservation capacity. These findings underscore the potential of ZnO/CA-Br-POD composites as multifunctional, eco-friendly materials for active food packaging applications.

Keywords

antimicrobial antioxidant black radish peroxidase calcium alginate food packaging immobilization zinc oxide NANOPARTICLES RELEASE ACID CLAY

WoS Categories

Food Science & Technology

Sustainable Development Goals

Clean Water and Sanitation

Citation

Journal: FOOD SCIENCE & NUTRITION
Year: 2025
Volume: 13
Issue: 8
ISSN: 2048-7177
DOI: 10.1002/fsn3.70694
WoS UID: WOS:001560864100033

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