Anti-Tumoral Effects of Pomegranate Fruit Membranes Across Different Phenotypes

Background and Objective: Pomegranate, a fruit from the cinnamon family, contains hundreds of seeds surrounded by membranes, which contribute to its unique taste and growth in temperate climates. This study aimed to investigate the anti-tumoral activities of membrane extracts from pomegranates of different phenotypes on lung cancer cell lines under in vitro conditions. Materials and Methods: Pomegranate fruits from three different phenotypes (Devedi & Otilde;i, Hicaz and Nuz sour) were harvested and the membranes were collected and dried. Solid-liquid extraction was used to obtain the extracts. The anti-tumoral effects of the membrane extracts were assessed using human healthy lung fibroblast cells and A549, a human Non-Small Cell Lung Cancer (NSCLC) cell line, as control and experimental groups, respectively. Malondialdehyde (MDA) was determined by measuring of lipid peroxidation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was measured for cytotoxic activity conducted on the cell lines. Data are presented as Mean +/- SEM from triplicate experiments, analyzed by One-way ANOVA with Tukey's post hoc test (GraphPad Prism 8.0), with p<0.05 considered significant. Results: The most effective compound in terms of cell viability was the Devedi & Otilde;i membrane extract. The MDA analysis further revealed that Nuz sour membrane extract exhibited the highest anti-tumoral activity. Conclusion: These findings suggest the potential of pomegranate membrane extracts as a source of anti-cancer agents, particularly for lung cancer therapy.

Journal

INTERNATIONAL JOURNAL OF PHARMACOLOGY

Year

2025

Volume

21

Issue

2

ISSN

1811-7775

eISSN

1812-5700

DOI

10.3923/ijp.2025.317.323

WoS UID

WOS:001502528000003