Antimutagenic and antimicrobial activity against pathogenic bacteria of endemic<i> Onobrychis</i><i> tournefortii</i> (Fabaceae) fruit extract and its anatomical characterisation

In this study, the anatomical definition, antimicrobial activity and antimutagenic effects of Onobrychis tournefortii were investigated. The antibacterial and antifungal activity of methanolic fruit extract of O. tournefortii (OtMFE) were investigated against various pathogen Gram (+) and Gram (-) bacteria and yeast. The findings demonstrated that this extract significantly inhibited the growth of several tested pathogenic microorganisms to varying degrees. The genotoxic effects in human lymphocyte cells in vitro were also assessed and confirmed by the micronucleus assay results. In addition, this study showed that O. tournefortii exhibits antigenotoxic activity against the mutagenicity of aflatoxin B1 (AFB1). This is the first report demonstrating that (OtMFE) has antigenotoxic activity against AFBi-induced DNA damage in vitro human lymphocyte cells using the micronucleus (MN) assay. The anatomical features of the fruit of the species, particularly the presence of parallel three-ellipsoid outlines, and the presence of macrosclerides and one-row osteosclerides in the seed coat can be counted among the distinguishing features of the species. This study is the first to investigate the anatomical, antimicrobial and antigenotoxic properties of the Turkish endemic O. tournefortii fruit, contributing to both knowledge about the genus and the development of pharmacological products.

Journal

COMPREHENSIVE PLANT BIOLOGY

Year

2025

Volume

49

Issue

1

Pages

39-48

eISSN

3042-3201

DOI

10.2298/CPB2501039U

WoS UID

WOS:001500919200004