MOLECULAR CHARACTERIZATION OF MUTATION REGIONS IN HERBICIDE-RESISTANT QUINOA (<i>Chenopodium quinoa Willd</i>.) MUTANT LINES

Quinoa can be considered as an alternative plant with its features being compatible with unfavorable climate and soil conditions. Moreover, the nutrient content of its seed is great. But there is not a selective herbicide for weeds in quinoa fields. This is a significant problem in the quinoa cultivation. Therefore, it is necessary to develop herbicide-resistant quinoa lines<br /> In the study, Tititcaca, a variety of quinoa was used. Sodium azide was used 1.5 mM for mutagenesis. Herbicide-resistant plants were determined by applying herbicide belonging to the IMI group in the M3 generation. These were named ET-6, ET-7, OT-11 and T-103. The 4 mutant lines obtained as a result of seed mutagenesis, and mutant line OT-11 plants occurred in the form of replacement of the cytosine nucleotide with the adenine nucleotide (C -> A) in the ALS gene. Line ET-6 plants, the thymine nucleotide was replaced with a guanine nucleotide (T -> G). The mutation occurring in the OT-11 and ET-6 genotypes was evaluated as a transversion-type mutation. A transition type mutation occurred in mutant line T-103 plants as well, and this mutation occurred in the form of substitution of thymine nucleotide with cytosine nucleotide (T -> C) at nucleotide 1114.<br /> The findings suggest that effective weed control in quinoa cultivation can be done effectively by developing varieties resistant to IMI group herbicides in quinoa plants. Herbicide resistance studies should be continued in the next generations with the ET-6, OT-11, and T-103 lines.

Journal

TURKISH JOURNAL OF FIELD CROPS

Year

2024

Volume

29

Issue

2

Pages

101-105

ISSN

1301-1111

DOI

10.17557/tjfc.1469935

WoS UID

WOS:001331409600003