Anti-Cataract Effect of the Traditional Aqueous Extract of Yerba Mate (<i>Ilex paraguariensis</i> A. St.-Hil.): An In Ovo Perspective
Oncu, Seyma, Becit-Kizilkaya, Merve, Bilir, Abdulkadir, Saritas, Alperen, Arikan-Soylemez, Evrim Suna, Koca, Halit Bugra, Firat, Fatma, Ugur-Kaplan, Afife Busra, Yilmaz, Mustafa Abdullah
Introduction: The therapeutic effect of different doses of the traditional aqueous extract of dried leaves of yerba mate (Ilex paraguariensis A. St.-Hil.) was investigated in an experimental cataract model in chicken embryos. Methods and Results: LC-MS/MS analysis allowed the identification and quantification of 53 metabolites. In the hydrocortisone-induced cataract model, lenses were examined morphologically after treatment and parameters related to oxidative stress (total antioxidant/oxidant status (TAS/TOS), glutathione (GSH), and malondialdehyde (MDA)) were evaluated. Antiproliferative cell nuclear antigen (PCNA) and caspase-3 H-scores were determined and crystallin alpha A (CRYAA) gene expression in the lenses was measured by RT-PCR. The degree of cataract decreased in all treatment groups. While there was no significant difference in TAS levels compared to the negative control, TOS, GSH, and MDA levels were dose-dependently regulated. Treatment groups other than the high-dose group regulated the decrease in PCNA and the increase in caspase-3. CRYAA gene expression increased significantly only at the lowest dose. Conclusion: YM, which is becoming increasingly popular as a traditional tea, showed a therapeutic effect on hydrocortisone-induced cataracts in chicken embryos at relatively low doses.
- Journal
- LIFE-BASEL
- Year
- 2024
- Volume
- 14
- Issue
- 8
- eISSN
- 2075-1729
- DOI
- 10.3390/life14080994
- WoS UID
- WOS:001307280900001