Ce₂O₃ nanoparticle synthesis, characterization, and application to callus formation and plant regeneration from mature embryo culture of wheat (<i>Triticum aestivum</i> L.)

Cerium oxide nanoparticles (Ce2O3-NPs) are widely used for their catalytic and oxidative properties, such as in diesel additives and potential oncology treatments. However, limited data exists on their impact on callus formation and plant regeneration in wheat. This study investigates the effects of different genotypes and Ce2O3-NPs concentrations on callus formation and plant regeneration in wheat (Triticum aestivum L.) using mature embryo culture. Our hypothesis was that Ce2O3-NPs would enhance callus and plant regeneration rates in a genotype-dependent manner. We used Murashige and Skoog (MS) medium with varying concentrations of Ce2O3-NPs (1-7 mg/L) to assess their effects. The K & imath;rik genotype showed the highest callus formation (7 mg/L Ce2O3-NPs), while the Rumeli genotype showed the lowest (1 mg/L Ce2O3-NPs). Embryogenic callus formation was the highest in K & imath;rik without Ce2O3-NPs and the lowest in Rumeli with 3 mg/L Ce2O3-NPs. Root and shoot formation rates in Rumeli were 16.03% and 51.40%, respectively, compared to 3.94% and 40.49% in K & imath;rik. The main outcomes demonstrated that 1 mg/L Ce2O3-NPs promoted root formation, while 7 mg/L was optimal for shoot formation. Genotypic effects on callus, root, and shoot formation, as well as plant regeneration, were significant. MS media with Ce2O3-NPs enhanced callus formation and regeneration in wheat tissue culture, highlighting the importance of genotype in these processes. Future research should explore the underlying mechanisms of Ce2O3-NPs' effects on plant tissue culture and extend the study to other plant species to validate these findings.

Journal

PLANT CELL TISSUE AND ORGAN CULTURE

Year

2024

Volume

158

Issue

3

ISSN

0167-6857

eISSN

1573-5044

DOI

10.1007/s11240-024-02842-1

WoS UID

WOS:001298791800002