Article 2025 · 6 citations

Utilizing Copper Nanoclusters as a Fluorescent Probe for Quantitative Monitoring of Doxorubicin Anticancer Drug

Polat, Muhammed Seyid, Nadaroglu, Hayrunnisa

Abstract

Monitoring the amount of chemotherapeutic drugs in biological fluids is extremely important for dose adjustment or control of side effects during the treatment process. In this study, copper nanoclusters (Cu NCs) were synthesized via a one-pot method using ammonium citrate as the reducing agent. Cu NCs exhibited bright blue fluorescence, good optical properties and outstanding photostability. The produced Cu NCs were characterized in detail by UV-vis absorption, fluorescence spectroscopy and transmission electron microscopy (TEM). The produced Cu NCs showed a high quantum yield of 0.97. A fluorescence system was used for doxorubicin (DOX) determination using Cu NCs as a nanoprobe. The presence of DOX decreased the fluorescence intensity of the CuNCs at 445 nm but increased the fluorescence intensity of the CuNCs at 619 nm. As a result, quantitative detection of DOX can be achieved by measuring the ratio of fluorescence intensities at 445 and 619 nm (F619/F445). The fluorescence quenching activity of the Cu NCs was determined to have a linear relationship with the amount of DOX anticancer drug in the range of 1-15 ppb, and the usability of the Cu NCs as a sensor for detection in biological fluids was demonstrated. It was determined that this method can be used to measure the amount of DOX in biological samples effectively.

Keywords

Cu NCs Pyrolysis method Doxorubicin Anticancer drugs Biological liquids GOLD NANOCLUSTERS PARTICLES SENSORS CYANIDE SWITCH

WoS Categories

Biochemical Research Methods Chemistry, Analytical Chemistry, Physical

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: JOURNAL OF FLUORESCENCE
Year: 2025
Volume: 35
Issue: 5
Pages: 3507-3518
ISSN: 1053-0509
eISSN: 1573-4994
DOI: 10.1007/s10895-024-03779-6
WoS UID: WOS:001242163300001

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