Bioinformatics approach combined with experimental verification reveals <i>OAS3</i> gene implicated in paclitaxel resistance in head and neck cancer
Caglar, Hasan Onur, Aytatli, Abdulmelik, Barlak, Neslisah, Karatas, Elanur Aydin, Tatar, Arzu, Sahin, Abdulkadir, Karatas, Omer Faruk
Background: This study aimed to identify a candidate gene associated with paclitaxel (PTX) resistance and to evaluate functionally its biological role in the PTX-resistant head and neck squamous cell carcinoma (HNSCC) cell lines and clinical specimens. Methods: Microarray data series containing samples of different types of cancers resistant to PTX were analyzed and then a candidate gene associated with PTX resistance was identified using various bioinformatics tools. After the suppression of the target gene expression, changes in cell viability and colony-forming ability were evaluated in PTX-resistant FaDu and SCC-9 cell lines. Results: Bioinformatics analyses of upregulated genes in PTX-resistant cancer cells indicated that OAS3 was associated with PTX resistance. The downregulation of OAS3 expression significantly reduced the viability and colony-forming capacity of PTX-resistant SCC-9 cells by inducing apoptosis and cell cycle arrest at G0/G1 phase. Conclusions: The therapeutic targeting of OAS3 may resensitize PTX-resistant HNSCC cells with high OAS3 expression to PTX treatment.
- Journal
- HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
- Year
- 2024
- Volume
- 46
- Issue
- 9
- Pages
- 2178-2196
- ISSN
- 1043-3074
- eISSN
- 1097-0347
- DOI
- 10.1002/hed.27803
- WoS UID
- WOS:001223938700001