Molecular characterization of some currants (<i>Ribes</i> species) from Türkiye using inter-primer binding site (iPBS) and simple sequence repeat (SSR) markers

This study was carried out to determine the genetic relationships and variation levels among currant genotypes and cultivars in T & uuml;rkiye. Ten currant cultivars and one hybrid Jostaberry cultivar, which are breeding products of Europe but are best known and have high commercial value in T & uuml;rkiye, were used. One black-fruited currant cultivar from T & uuml;rkiye was included. In addition, 14 currant genotypes naturally grown in T & uuml;rkiye were used. In this study, 14 retrotransposon-based inter-primer binding site (iPBS) and 10 microsatellite-based simple sequence repeat (SSR) markers were used for a total of 26 currant samples. By evaluating the allele profiles obtained from PCR and capillary electrophoresis with iPBS and SSR primers, the size range, average number of alleles, total number of alleles, number of polymorphic alleles, polymorphism rate, heterozygosity, polymorphism information content, marker index and discriminating power were determined successfully. In addition, unweighted pair group method with arithmetic mean (UPGMA) dendrograms and principal coordinate analysis (PCoA) were constructed and performed. The amounts of amplification product produced using iPBS and SSR markers for currant species with different fruit colours were also compared. In this study, it was proven that the level of genetic relatedness among currant cultivars and genotypes can be determined with high accuracy by iPBS markers. Moreover, the use of iPBS markers in currants was performed for the first time in this study. On the other hand, with the preferred SSR markers, the level of genetic relatedness among currants was successfully determined.

Journal

GENETIC RESOURCES AND CROP EVOLUTION

Year

2025

Volume

72

Issue

1

Pages

445-461

ISSN

0925-9864

eISSN

1573-5109

DOI

10.1007/s10722-024-01987-4

WoS UID

WOS:001208719100001