Article 2024 · 18 citations

A Comprehensive Study on Peppermint Oil and Cinnamon Oil as Nanoemulsion: Preparation, Stability, Cytotoxicity, Antimicrobial, Antifungal, and Antioxidant Activity

Ozakar, Emrah, Alparslan, Levent, Adiguzel, M. Cemal, Torkay, Gulsah, Baran, Alper, Bal-ozturk, Ayca, Sevinc-ozakar, Rukiye

Abstract

Background Recent studies have shown that nanoemulsions prepared with essential oils have significant antimicrobial potential against multidrug-resistant pathogens due to increased chemical stability. Nanoemulsion also promotes controlled and sustained release, which increases their bioavailability and efficacy against multidrug-resistant bacteria.Objective This study aimed to investigate the antimicrobial, antifungal, antioxidant, and cytotoxicity properties of cinnamon essential oil and peppermint essential oil as nanoemulsions compared to pure forms. For this purpose, analyses of the selected stable nanoemulsions were carried out.Methods The droplet sizes and zeta potentials of peppermint essential oil nanoemulsions and cinnamon essential oil nanoemulsions were found to be 154.6 +/- 1.42 nm and -17.1 +/- 0.68 mV and 200.3 +/- 4.71 nm and -20.0 +/- 0.81 mV, respectively. Although the amount of essential oil used in nanoemulsions was 25% w/w, antioxidant and antimicrobial activities were found to be more effective compared to pure essential oils.Results In cytotoxicity studies on the 3T3 cell line, both essential oil nanoemulsions showed higher cell viability than pure essential oils. At the same time, cinnamon essential oil nanoemulsions exhibited a higher antioxidant property than peppermint essential oil nanoemulsions and showed superiority in the antimicrobial susceptibility test conducted against four bacteria and two fungi. Cell viability tests determined that cinnamon essential oil nanoemulsions showed considerably higher cell viability compared to pure cinnamon essential oil.Conclusion These findings indicated that the prepared nanoemulsions in the current study might positively influence the dosing regimen and clinical outcomes of antibiotic therapy.

Keywords

Cinnamon oil peppermint oil nanoemulsion stability antioxidant antimicrobial cell viability IN-VITRO EVALUATION PHYSICAL-PROPERTIES ANTIBACTERIAL ACTIVITY FORMULATION SURFACTANT EMULSIONS NANOPARTICLES PERFORMANCE CHITOSAN

WoS Categories

Pharmacology & Pharmacy

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: CURRENT DRUG DELIVERY
Year: 2024
Volume: 21
Issue: 4
Pages: 603-622
ISSN: 1567-2018
eISSN: 1875-5704
DOI: 10.2174/1567201820666230612123011
WoS UID: WOS:001138071900009

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