Investigating effects of locally applied boric acid on fracture healing with and without low-level laser therapy

This study aimed to investigate the effects on fracture healing of locally applied boric acid ( BA) with and without low-level laser therapy (LLLT). A unicortical femoral defect was surgically created on the anterolateral surface of proximal femur of each subject. The subjects, totaling 56 Wistar albino rats, were randomly allocated into four groups (n = 14 each): control, LLLT (lambda = 905 mu m, 10,000 Hz, 25 mW, and peak power 25 W), BA (40 mg/kg), and BA + LLLT groups. On the 30th day, the highest radiological score was recorded for the BA + LLLT group (3.63 [2-4]), followed by the BA (3.38 [2.75- 3.75]), control (3 [2-3.25]), and LLLT ( 2.5 [1.25- 3]) groups. On days 15 and 30 post-surgery, malondialdehyde levels were significantly lower among the BA + LLLT group compared to the control group (p < 0.001). On day 30, superoxide dismutase, catalase, and alkaline phosphatase levels were highest in the BA + LLLT group compared to the control group (p < 0.001). When the histopathological, immunofluorescence, and immunohistochemical findings on the 15th and 30th days were compared with the control group, a statistically significant difference was found for the BA and BA + LLLT groups (p. 0.05). This study suggests that locally applied BA with LLLT may accelerate fracture healing.

Journal

LASERS IN MEDICAL SCIENCE

Year

2022

Volume

38

Issue

1

ISSN

0268-8921

eISSN

1435-604X

DOI

10.1007/s10103-022-03695-w

WoS UID

WOS:001056594700005