Pathological and molecular investigation of canine distemper virus: Phylogenetic analysis of co-circulating genetic lineages in Turkiye

Canine distemper virus (CDV) is a highly contagious virus that infects a wide variety of animals of carnivore species and may cause manifestations from subclinical infection to fatal disease. In this study, dogs clinically suspected having distemper were examined by reverse transcriptase-polymerase chain reaction (RT-PCR), histopathology and immuno-histochemistry. By histopathological examination, characteristic intracytoplasmic and/or intranuclear inclusion bodies were observed in the lung, stomach, small intestine, liver, kidney, spleen and central nervous system. Interstitial and broncho-interstitial pneumonia, gastroenteritis and encephalitis were revealed. CDV antigens were detected in all tissues with characteristic histopathological findings. The antigens were more abundant in the bronchial and bronchiolar epithelium and in the syntitial cells. Phylogenetic analyses were performed using the PCR-amplified partial sequences of the genes encoding the viral heamagglutinin and fusion proteins. The phylogenetic trees showed that the newly determined sequences were diverse and clustered within different lineages of the European or the Arctic strains.

Journal

ACTA VETERINARIA HUNGARICA

Year

2023

Volume

71

Issue

1

Pages

54-64

ISSN

0236-6290

eISSN

1588-2705

DOI

10.1556/004.2023.00809

WoS UID

WOS:001027645200010