Article 2023 · 4 citations

α-tocopherol ameliorates copper II oxide nanoparticles-induced cytotoxic, biochemical, apoptotic, and genotoxic damages in the rainbow trout gonad cells-2 (RTG-2) culture

Abstract

We investigated the effects of alpha-tocopherol on oxidative stress-caused damage caused by copper II oxide nano-particles (CuO NPs) on Oncorhynchus mykiss gonadal cells (RTG-2) for 24 and 48 h. alpha-Tocopherol reversed the cell death and alterations in the expressions of genes such as sod1, gpx1a, gpx4b, and igf2 caused by CuO NPs; it also supported the expressions of cat, igf1, and gapdh genes caused by CuO NPs for 24 h and promoted alterations in the expressions of the sod2, gh1, and igf1 genes for 48 h. Additionally, alpha-tocopherol reversed the caspase 3/7 activity increased by CuO NPs for 24 h and supported it's decrease for 48 h. alpha-Tocopherol supported the increase in tail DNA (%) affected by CuO NPs for 24 h and reversed it for 48 h. Therefore, alpha-tocopherol may have the potential to protect against cellular alterations induced by CuO NPs in a time-dependent manner.

Keywords

alpha-Tocopherol CuO NPs Cytotoxicity Gene expressions Caspase 3/7 activity DNA damage RTG-2 cells VITAMIN-E ANTIOXIDANT TOXICITY SYSTEM GENES CUO MECHANISMS ASSAY

WoS Categories

Environmental Sciences Pharmacology & Pharmacy Toxicology

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Year: 2023
Volume: 101
ISSN: 1382-6689
eISSN: 1872-7077
DOI: 10.1016/j.etap.2023.104168
WoS UID: WOS:001019734600001

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