Article 2024 · 4 citations

Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway

Koc, Kubra, Ozek, Nihal Simsek, Aysin, Ferhunde, Demir, Ozlem, Yilmaz, Asli, Yilmaz, Mehmet, Geyikoglu, Fatime, Erol, Huseyin Serkan

Abstract

This study investigates the protective role of Hispidulin on acute respiratory distress syndrome (ARDS) in rats. Rats were divided into three groups: control, ARDS, ARDS+ Hispidulin. The ARDS models were established by injecting rats with oleic acid. Hispidulin (100 mg/kg) was injected i.p. an hour before ARDS. Myeloperoxidase (MPO), Interleukin-8 (IL-8), Mitogen-activated protein kinases (MAPK), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD), Glutathione (GSH), and Angiotensin-converting enzyme (ACE) were determined by ELISA. Tumor necrosis factor-alpha (TNF-alpha) expression was described by RT-qPCR. Caspase-3 immunostaining was performed to evaluate apoptosis. Compared with the model group, a significant decrease was observed in the MPO, IL-8, MAPK, ACE, LPO levels, and TNF-alpha expression in the ARDS+ Hispidulin group. Moreover, reduced caspase-3 immunoreactivity and activity of ACE were detected in the Hispidulin+ARDS group. The protective effect of Hispidulin treatment may act through inhibition of the ACE activity and then regulation of inflammatory cytokine level and alteration of apoptosis.

Keywords

Hispidulin lung injury with ARDS ACE activity RESPIRATORY-DISTRESS-SYNDROME ACUTE LUNG INJURY OXIDATIVE STRESS FATTY-ACIDS PATHOGENESIS MICE APOPTOSIS

WoS Categories

Environmental Sciences Public, Environmental & Occupational Health

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
Year: 2024
Volume: 34
Issue: 2
Pages: 755-766
ISSN: 0960-3123
eISSN: 1369-1619
DOI: 10.1080/09603123.2023.2166023
WoS UID: WOS:000909864500001

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