Purification and Characterization of Lipoxygenase from Walnuts (Juglans Regia) and Investigation of the Effects of Some Phenolic Compounds on the Activity

This study reports the purification and characterization of lipoxygenase protein from walnut (Juglans regia). Lipoxygenases (LOX) are the key enzyme of germination in plants. Purification of LOX was 62,28 fold in 68 % yield with a specific activity of 24.29 U/mg using ammonium sulfate precipitation and DEAE-Sepharose Fast Flow Ion Exchange Column. The optimum pH value of the enzyme was determined as pH 7 and the optimum temperature value as 30 degrees C. It was determined that morin hydrate, p-coumaric acid, syringaldehyde and vanillic acid inhibited LOX, and their IC50 values were 304 mu M, 233 mu M, 643.2 mu M and 218.1 mu M respectively. It has been concluded that walnut, which is used frequently in many parts of the world, is a good source of LOX and this study can be a guide for studies in medicine, food industry, cosmetics and pharmacy.

Journal

CHEMISTRYSELECT

Year

2022

Volume

7

Issue

46

ISSN

2365-6549

DOI

10.1002/slct.202203961

WoS UID

WOS:000893842400001