Article 2021 · 15 citations

The cytotoxic and oxidative effects of restorative materials in cultured human gingival fibroblasts

Celik, Neslihan, Binnetoglu, Damla, Ozakar Ilday, Nurcan, Hacimuftuoglu, Ahmet, Seven, Nilgun

Abstract

The aim of this study was to evaluate the cytotoxic and oxidative effects of the most commonly used dental restorative materials on human gingival fibroblast cells (HGFCs). HGFCs were obtained from healthy individuals. The tested restorative materials were a microhybrid resin based composite, a compomer resin, a glass ionomer cement, and an amalgam alloy. One hundred eight cylindirical samples, 10mm in diameter and 2mm in height, were prepared according to ISO 10993-12:2002 specifications (n=9 in the tested subgroups). Freshly prepared and aged samples in artificial saliva at 37 degrees C (7 and 21 d) were placed into well plates and incubated. Wells without dental materials were constituted as the control group. After 72h incubation period, cytotoxicity was determined using the neutral red (NR) assay. Oxidative alterations were assessed using total antioxidant capacity (TAC) and total oxidant status (TOS) assay kits. Data were analyzed using the ANOVA and LSD post hoc tests. All tested materials led to significant decreases in the cell viability rates (33-73%) compared to the control group. Glass ionomer and resin composite were found to be more cytotoxic than amalgam alloy and compomer. The highest TAC level was observed in glass ionomer after seven-day aging and these changes prevented an increase in TOS levels. Increases in TAC levels after seven-day aging in all groups exhibited significant differences with freshly prepared samples (p<0.05). In all material groups, TOS levels of freshly prepared samples differed statistically and significantly from samples aged for 7 and 21 d (p<0.05). The data obtained suggested that all the tested materials exhibited cytotoxic and pro-oxidant features. Freshly prepared samples caused higher TOS levels. However, oxidant status induced by materials decreased over time.

Keywords

Cytotoxicity neutral red restorative dental materials oxidative stress END FILLING MATERIALS IN-VITRO CYTOTOXICITY ORAL FIBROBLASTS BIOCOMPATIBILITY MONOMERS GENOTOXICITY PRINCIPLES CHILDREN AMALGAM STRESS

WoS Categories

Chemistry, Multidisciplinary Pharmacology & Pharmacy Toxicology

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: DRUG AND CHEMICAL TOXICOLOGY
Year: 2021
Volume: 44
Issue: 5
Pages: 502-507
ISSN: 0148-0545
eISSN: 1525-6014
DOI: 10.1080/01480545.2019.1620265
WoS UID: WOS:000885440500006

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