Article 2022 · 6 citations

Production of Endoglucanase by <i>Exiguobacterium mexicanum</i> OB24 Using Waste Melon Peels as Substrate

Baltaci, Mustafa O., Omeroglu, Mehmet A., Albayrak, Seyda, Adiguzel, Gulsah, Adiguzel, Ahmet

Abstract

Millions of tons of agricultural waste are produced globally every year. A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source. To use this important resource, its stubborn structure must be broken down. Rumen bacteria are regarded as unique for this job. Therefore, firstly endoglucanase producing rumen bacteria was isolated and the bacteria with the best activity (OB24) were identified by molecular methods (165 rRNA gene squencing). As a result of the sequence analysis, it was determined that isolate belonged to Exiguobacterium mexicanum. Then, by optimizing the culture conditions, the enzyme production potential was increased. The optimal conditions were determined as 50 g/L MPP, 2g/L yeast extract, 60 h incubation time, pH: 6.0, and 40 degrees C temperature. Under optimized conditions the enzyme activity increased approximately 3.8-fold.

Keywords

Endoglucanase production waste melon peel Exiguobacterium mexicanum OB24 agricultural wastes ENZYME-PRODUCTION NITROGEN-SOURCE OPTIMIZATION RUMEN FERMENTATION BACTERIA VALORISATION PRETREATMENT FRUIT

WoS Categories

Multidisciplinary Sciences

Sustainable Development Goals

Responsible Consumption and Production Affordable and Clean Energy

Citation

Journal: ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Year: 2022
Volume: 94
ISSN: 0001-3765
eISSN: 1678-2690
DOI: 10.1590/0001-3765202220220151
WoS UID: WOS:000853999600001

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