Article 2022 · 1 citations

Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

Abstract

This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3, Cas-8, Cas-9, Bax, Bcl-2, LC3B, and Beclin-1 expression with real-time PCR. The expression levels of Beclin-1 and LC3B, autophagy markers, were significantly up-regulated in comparison with the control group (p < 0.001). The expression levels of apoptotic markers Cas-3, Cas-8, Cas-9 and Bax were slightly up-regulated, but Bcl-2 was slightly down-regulated in contrast to the control group (p < 0.05). Therefore, the autophagy markers were more activated than apoptotic markers in dogs with acute neurological signs. In conclusion, autophagy takes part in the pathogenesis of demyelination in canine distemper. Knowing this may be helpful to create new therapeutic strategies, such as new effective antiviral medicines.

Keywords

apoptosis autophagy canine distemper real-time PCR ENCEPHALOMYELITIS MORBILLIVIRUS MECHANISMS EXPRESSION MULTIPLE FUSION DEATH LC3

WoS Categories

Veterinary Sciences

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: ACTA VETERINARIA-BEOGRAD
Year: 2022
Volume: 72
Issue: 2
Pages: 195-206
ISSN: 0567-8315
eISSN: 1820-7448
DOI: 10.2478/acve-2022-0016
WoS UID: WOS:000819713900005

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