Determination of triacylglycerols in milk fat from different species using UPLC-Q-TOF-MS
Karrar, Emad, Ahmed, Isam A. Mohamed, Huppertz, Thom, Oz, Fatih, Wei, Wei, Wang, Xingguo
Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC-Q-TOF-MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC-Q-TOF-MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.(c) 2022 Elsevier Ltd. All rights reserved.
- Journal
- INTERNATIONAL DAIRY JOURNAL
- Year
- 2022
- Volume
- 133
- ISSN
- 0958-6946
- eISSN
- 1879-0143
- DOI
- 10.1016/j.idairyj.2022.105405
- WoS UID
- WOS:000812258500005