Article 2022 · 7 citations

Therapeutic effects of apocynin on ovarian ischemia-reperfusion induced lung injury

Tanyeli, Ayhan, Erdogan, Derya Guzel, Comakli, Selim, Polat, Elif, Guler, Mustafa Can, Eraslan, Ersen, Doganay, Songul

Abstract

Ovarian ischemia-reperfusion (I-R) injury may damage remote organs, including the lungs. We investigated whether apocynin, a NADPH oxidase inhibitor, might protect against ovarian I-R induced apoptosis in the lungs of rats. Bilateral ovarian I-R was induced for 3 h, then apocynin was applied at two concentrations. Lung tissue was evaluated using spectrophotometric and immunohistochemical methods. We found that I-R increased total oxidant status (TOS), oxidative stress index (OSI) and myeloperoxidase (MPO) levels, and immunostaining of nuclear factor kappa-B (NF-kappa B), light chain 3B (LC3B), interleukin 1-beta (IL-1 beta), caspase-3 and tumor necrosis factor-alpha (TNF-alpha), but decreased superoxide dismutase (SOD) values. Apocynin application to I-R injured rats enhanced recovery of lung tissue oxidants and improved both histology and frequency of apoptosis.

Keywords

Apocynin apoptosis ischemia lung injury oxidative damage rats reperfusion GERM-CELL APOPTOSIS ISCHEMIA/REPERFUSION INJURY OXIDATIVE STRESS ENDOPLASMIC-RETICULUM AUTOPHAGY INFLAMMATION INHIBITION PATHWAY TISSUE DEATH

WoS Categories

Biotechnology & Applied Microbiology Cell Biology

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: BIOTECHNIC & HISTOCHEMISTRY
Year: 2022
Volume: 97
Issue: 7
Pages: 536-545
ISSN: 1052-0295
eISSN: 1473-7760
DOI: 10.1080/10520295.2022.2036368
WoS UID: WOS:000754980600001

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