Anticancer effects of novel NSAIDs derivatives on cultured human glioblastoma cells
Ozdemir, Ozlem, Marinelli, Lisa, Cacciatore, Ivana, Ciulla, Michele, Emsen, Bugrahan, Di Stefano, Antonio, Mardinoglu, Adil, Turkez, Hasan
Several epidemiologic, clinical and experimental reports indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) could have a potential as anticancer agents. The aim of this study was the evaluation of cytotoxic potential in human glioblastoma cells of novel synthesized NSAID derivatives, obtained by linking, through a spacer, alpha-lipoic acid (ALA) to anti-inflammatory drugs, such as naproxen (AL-3, 11 and 17), flurbiprofen (AL-6, 13 and 19) and ibuprofen (AL-9, 15 and 21). The effects on the level of gene expression were also determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. According to our results, NSAID derivatives exhibited concentration dependent cytotoxic effects on U87-MG cell line when compared with the control group. Moreover, treatment of the most active compounds (AL-3, AL-6 and AL-9) caused upregulation of tumor suppressor gene PTEN and downregulation of some oncogenes such as AKT1, RAF1 and EGFR. In conclusion, our results revealed that AL-3, AL-6 and AL-9 could be suitable candidates for further investigation to develop new pharmacological strategies for the prevention of cancer.
- Journal
- ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
- Year
- 2021
- Volume
- 76
- Issue
- 7-8
- Pages
- 329-335
- ISSN
- 0939-5075
- eISSN
- 1865-7125
- DOI
- 10.1515/znc-2020-0093
- WoS UID
- WOS:000672580800007