Article 2022 · 32 citations

The effect of brimonidine and proparacaine on metabolic enzymes: Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase

Caliskan, Busra, Ozturk Kesebir, Arzu, Demir, Yeliz, Akyol Salman, Ilknur

Abstract

Oxidative stress is to upregulate the pentose phosphate pathway (PPP). The PPP consists of two functional branches, glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconaste dehydrogenase (6PGD). Glutathione reductase (GR) has a significant role in catalyzing an oxidized glutathione form into a reduced form. The purpose of this study is to investigate the effects of brimonidine and proparacaine on the activity of 6PGD, G6PD, and GR enzymes purified from human erythrocytes. Brimonidine displayed considerable inhibition profile against G6PD with IC50 value and K-I constant of 29.93 +/- 3.56 and 48.46 +/- 0.66 mu M, respectively. On the other hand, proparacaine had no inhibitory effect against G6PD. K-I values were found to be 66.06 +/- 0.78 and 811.50 +/- 11.13 mu M for brimonidine and proparacaine, respectively, for 6PGD. K-I values were found to be 144.10 +/- 2.01 and 1,654.00 +/- 26.29 mu M for brimonidine and proparacaine, respectively, for GR. Herein, also in silico molecular docking studies were performed between drugs and enzymes.

Keywords

glucose-6-phosphate dehydrogenase 6-phosphogluconate dehydrogenase glutathione reductase brimonidine proparacaine IN-VITRO INHIBITION DRUGS ANTIBIOTICS GLAUCOMA MODULATION SODIUM SILICO CELLS LENS

WoS Categories

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology

Sustainable Development Goals

Good Health and Well-being

Citation

Journal: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Year: 2022
Volume: 69
Issue: 1
Pages: 281-288
ISSN: 0885-4513
eISSN: 1470-8744
DOI: 10.1002/bab.2107
WoS UID: WOS:000612484900001

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