Article Early Access 2025 · 0 atıf

Astaxanthin and omega-3 fatty acids enhance endometrial receptivity in a rat model of polycystic ovary syndrome

Bal Tastan, Tugba, Selli, Jale, Toktay, Erdem, Ustundag, Hilal, Ugan, Rustem Anil

Özet

Polycystic ovary syndrome (PCOS) adversely affects endometrial receptivity and fertility outcomes. This study investigated whether combining high-dose antioxidants (astaxanthin or omega-3 fatty acids) with metformin improves endometrial receptivity markers, reduces oxidative stress, and normalizes hormone profiles in a PCOS rat model compared to metformin alone or no treatment. Fifty-six female Sprague-Dawley rats (200-250g) were divided into seven groups: Control, PCOS, PCOS + Met, and groups receiving metformin plus either astaxanthin (PCOS + Met + AX5 or PCOS + Met + AX10) or omega-3 (PCOS + Met + OMG400 or PCOS + Met + OMG1000). PCOS was induced using letrozole for 21 days, followed by one week of treatment. After establishing endometrial receptivity, uterine tissues were analyzed histopathologically, immunohistochemically, and biochemically on day 5. The PCOS group demonstrated significant increases in body weight and oxidative stress markers (malondialdehyde) compared to controls (p < 0.001). High-dose antioxidant supplementation (PCOS + Met + AX10 and PCOS + Met + OMG1000) significantly improved these parameters (p < 0.001). Immunohistochemically, integrin beta 3 expression increased while mucin 1 (MUC-1) decreased in high-dose groups compared to PCOS (p < 0.001). The LH:FSH ratio normalized to control levels in PCOS + Met + AX10 and PCOS + Met + OMG1000 groups. Histological examination revealed enhanced endometrial morphology and increased pinopode-like structure formation in antioxidant-supplemented groups. High-dose astaxanthin and omega-3 fatty acid supplementation, combined with metformin, may enhance endometrial receptivity in PCOS by reducing oxidative stress and modulating key implantation markers. This therapeutic approach could potentially improve fertility outcomes in women with PCOS.

Anahtar Kelimeler

Astaxanthin Endometrial receptivity Omega-3 Oxidative stress Polycystic ovary syndrome OXIDATIVE STRESS SYNDROME PCOS ANTIOXIDANTS EXPRESSION PINOPODES OMEGA-3 IMPLANTATION INFLAMMATION INTERPLAY MARKER

WoS Kategorileri

Pharmacology & Pharmacy

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam Toplumsal Cinsiyet Eşitliği

Künye

Dergi: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Yıl: 2025
ISSN: 0028-1298
eISSN: 1432-1912
DOI: 10.1007/s00210-025-04808-2
WoS UID: WOS:001649964800001

Yayını aç (DOI)