Decoding serum N-Glycome signatures across COVID-19 symptomatic stages
Yaman, Mehmet Emrah, Kayili, Haci Mehmet, Avci, Izzet, Aksakal, Alperen, Kocak, Omer Faruk, Atakay, Mehmet, Salih, Bekir
SARS-CoV-2 infection causes a wide spectrum of clinical manifestations, ranging from asymptomatic cases to fatal outcomes. Although significant progress has been made in understanding SARS-CoV-2, the identification of reliable biomarkers for early risk assessment and disease severity prediction remains an unmet clinical need. In this study, we characterized the serum N-glycome profiles of a cohort comprising COVID-19 patients with mild to severe symptoms and healthy controls. After enzymatic deglycosylation, serum samples were analyzed using HILIC-FLD-QTOF-MS. In case-control comparisons, COVID-19 patients exhibited a significant decrease in oligomannose and hybrid-type glycans, along with an increase in tetra-antennary and tetra-galactosylated structures. In addition, four N-glycan structures were identified as having diagnostic potential to distinguish COVID19 cases from healthy controls. When comparing severely symptomatic COVID-19 patients to those with mild symptoms and healthy controls, a significant increase was observed in antennary fucosylated N-glycans (SLex). Moreover, ROC analysis demonstrated that an isomer of Hex5HexNAc4Neu5Ac had strong diagnostic potential (AUC > 0.8) in distinguishing severely symptomatic COVID-19 patients from healthy controls. Our study reveals a novel association between antennary fucosylation and an isomer of Hex5HexNAc4Neu5Ac in severe COVID-19, highlighting its potential relevance for biomarker discovery.
- Dergi
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
- Yıl
- 2025
- Cilt
- 1263
- ISSN
- 1570-0232
- eISSN
- 1873-376X
- DOI
- 10.1016/j.jchromb.2025.124710
- WoS UID
- WOS:001530478000001