Article 2025 · 1 atıf

Deciphering the genotoxic and cytotoxic properties of teicoplanin: a combined laboratory and computational investigation

Berber, Ahmet Ali, Aliravci, Isil Deniz, Kenanoglu, Nihan Akinci, Demir, Sefika Nur

Özet

In this study, the mutagenicity and carcinogenicity of the teicoplanin antibiotic were first investigated using the Vega Hub and Toxtree software through in silico prediction. The cytotoxic and genotoxic effects were evaluated using in vitro assays, including the mitotic index (MI), micronucleus (MN), nuclear division index (NDI), and Comet Assay (CA) in human lymphocytes. In the in vitro studies, both 24-hour and 48-hour exposures were conducted for MI, and teicoplanin significantly decreased MI compared to the control at all concentrations. In addition, a significant increase was detected in the MN frequency compared to the negative control at all concentrations. In the Comet assay, tail length significantly increased compared to the control at all concentrations except for 5.6 mu g/mL, while tail moment and comet tail intensity significantly increased at all concentrations compared to the control. In conclusion, within the concentration range used in this study, teicoplanin was found to have cytotoxic and genotoxic effects.

Anahtar Kelimeler

Genotoxicity cytotoxicity micronucleus mitotic index comet teicoplanin in silico DNA-DAMAGE ASSAY MUTAGENICITY ANTIBIOTICS REPAIR MODELS

WoS Kategorileri

Chemistry, Multidisciplinary Pharmacology & Pharmacy Toxicology

Sürdürülebilir Kalkınma Amaçları

Sağlık ve Kaliteli Yaşam

Künye

Dergi: DRUG AND CHEMICAL TOXICOLOGY
Yıl: 2025
Cilt: 48
Sayı: 5
Sayfa: 1015-1024
ISSN: 0148-0545
eISSN: 1525-6014
DOI: 10.1080/01480545.2025.2502446
WoS UID: WOS:001484065700001

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