Combination of magnetic hyperthermia and gene therapy for breast cancer
Solak, Kubra, Arslan, Seyda Yildiz, Acar, Melek, Turhan, Fatma, Unver, Yagmur, Mavi, Ahmet
This study presented a novel breast cancer therapy model that uses magnetic field-controlled heating to trigger gene expression in cancer cells. We created silica- and amine-modified superparamagnetic nanoparticles (MSNP-NH2) to carry genes and release heat under an alternating current (AC) magnetic field. The heat-inducible expression plasmid (pHSP-Azu) was designed to encode anti-cancer azurin and was delivered by magnetofection. MCF-7 cells demonstrated over 93% cell viability and 12% transfection efficiency when exposed to 75 mu g/ml of MSNP-NH2, 3 mu g of DNA, and PEI at a 0.75 PEI/DNA ratio (w: w), unlike non-tumorigenic cells (MCF-10 A). Magnetic hyperthermia (MHT) increased azurin expression by heat induction, leading to cell death in dual ways. The combination of MHT and heat-regulated azurin expression induced cell death, specifically in cancer cells, while having negligible effects on MCF-10 A cells. The proposed strategy clearly shows that simultaneous use of MHT and MHT-induced azurin gene expression may selectively target and kill cancer cells, offering a promising direction for cancer therapy.
- Dergi
- APOPTOSIS
- Yıl
- 2025
- Cilt
- 30
- Sayı
- 1-2
- Sayfa
- 99-116
- ISSN
- 1360-8185
- eISSN
- 1573-675X
- DOI
- 10.1007/s10495-024-02026-4
- WoS UID
- WOS:001336263500002